Nikon Microscope ECLIPSE E800 Instructions Manual
The coarse focus stopper marks the stage position at which the specimen is in focus by restricting the movement of the coarse focus knob. (Movement of the stage by the fine focus knob is not restricted.) Once the coarse focus stopper is clamped in place, the coarse focus knob cannot be used to move the stage any higher. In effect, once the coarse focus knob is clamped in place at the focus position, a rough focus can be attained the next time simply by turning the coarse focus knob as far as it will go. This feature is convenient when viewing similar specimens one after another. If the coarse focus stopper is not being used, the coarse focus stopper ring should be loosened as far as it will go (by turning it as far as it will go in the direction opposite to that indicated by the arrow on the base of the microscope.)
Using the coarse focus stopper With the specimen in focus, turn the coarse focus stopper ring as far as it will go in the direction indicated by the arrow on the base of the microscope (about 270°). The coarse focus stopper is now clamped in place. When changing the specimen, lower the stage by turning only the coarse focus knob. After changing the specimen, gently raise the stage by turning only the coarse focus knob as far as it will go. The specimen should be roughly in focus when the stage has been raised as far as it will go; use the fine focus knob to bring the specimen into perfect focus.
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