Myocyte Test Apparatus Models 803B Instruction Manual
The 803B Myocyte test apparatus was designed to enable physiology researchers to easily test myocytes with an ASI series 312C motor and a 400A series force transducer. The 803B consists of a 6-well aluminum bath plate with a glass bottom on all 6 wells, 2 TEC heater/coolers, water-cooled heat sink and an ASI model 825A Thermometer/TEC controller (see separate 825A manual for details on operating the temperature controller). All parts are manufactured from corrosion resistant materials (stainless steel, anodized aluminum and Delrin).
The 803B also includes a large cell mounting area located in front of the 6 working wells. The cell mounting area accepts a standard 3”x1”x1mm microscope slide. The upper surface of the microscope slide is flush with the top surface of the plate to allow easy transfer of mounted cells from the mounting slide to the working wells. The cell is normally glued between the output tube of the force transducer and the motor arm of the 312C Length Controller. The apparatus mounts on the surface of a microscope XY translation stage and is held in place with four 3mm flat head screws. In operation the desired bath is selected by moving the 803B bath plate relative to the length controller and force transducer using the microscope’s XY translation stage.
The bath plate is manufactured from anodized aluminum and has six round wells machined in its upper surface. This number allows for test protocols involving multiple solutions without the need to empty and re-fill the baths. All baths have a glass bottom to allow for microscope observation of the cell or for sarcomere length measurement. The glass on the bottom of the plate is a standard 1 ½ microscope cover slip that measures 22×40mm. The aluminum plate is coated with a special hard anodized coating with polymer impregnated in the surface. This ensures that the surface is inert as well as corrosion resistant.
Get pdf Myocyte Test Apparatus Models 803B Instruction Manual
