LU-CB1 Leiden Micro-Incubator User’s Manual
The LU-CB1 is a simple micro-CO2-incubator designed for use on the microscope stage of a inverted microscope.The micro-incubator is easy to use and offers a handy tool for the culture of cells under the microscope.The performance of the LU-CB1 com- pares well with that of a conventional CO2-microincubator. A standard 35 mm plastic or glass culture dish can be placed in the micro-incubator. The culture medium is then covered by a gas permeable layer of mineral oil, which protects the culture from the environment without affecting culture conditions,allowing easy cell manipulation under microscopical control.
Micro-Incubator Construction
The LU-CB1 Leiden Micro-Incubator consists of:
• 3 torroidal rings anodized with black aluminum-oxide film
• Two-turns of cantal or nichrome wire wound around the middle ring and connected to external 1 mm jacks
• Gas perfusion inlet
• Polyvinylchloride (PVC) support ring with magnetic base (metal top) to allow mounting of thermistor probes or other devices with magnetic holders around the incubator.(Support ring is fitted with wells that can be used as reference electrode wells for electrophysiological experiments and two screws to hold the micro-incubator in place.)
• 3 (PVC) insulating rings to avoid contact with the insert plate of the micro- scope stage and to prevent heat loss.
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