Multiple Wavelength Multiviewer Operation Manual
The Multiviewer is a complete system for multispectral imaging. It allows two images to be collected simultaneously using Princeton Instruments CCD or ICCD cameras. It features high throughput dichroic optics and UV wavelength operation as an option. Princeton Instruments multiple wavelength Multiviewer system offers the following features:
• One image projected in dual wavelengths simultaneously on a single CCD or ICCD
• No filter wheel delays and no shot-to-shot variation
• No parallax. All images are taken from the same perspective
• UV operation to 200 nm (optional)
• High throughput dichroic optics
• Low stray light
Performance Considerations
The Multiviewer design allows an image to be acquired at two different wavelengths simultaneously. With a gated intensified camera, both images can be acquired in as little as 5 nanoseconds. This means that even transient events can now be recorded at multiple wavelengths. This eliminates problems of shot-to-shot variability that occur when recording a transient event at multiple wavelengths by recording successive occurrences. With a Multiviewer, the complete multispectral image is acquired in a single shot.
Even for slowly moving subjects, simultaneous imaging provides an advantage. While the morphology of a subject may not change very quickly, the intensity at an individual pixel can change abruptly due to slow movement of a sharp edge across the pixel. Ratio images created from successive frames taken at different wavelengths often contain artifacts due to this effect. Simultaneous dual-wavelength imaging eliminates this ambiguity.
Whether a system is designed for operation on transient or slowly changing subjects, the elimination of moving filter systems improves overall system reliability. Since the Multiviewer has no moving parts, the reliability is much higher. The passive design also requires no computer control and has no power requirements.
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