USING THE HP 6890/5973 GC-MS
As long as there is room in the autosampler tray, more samples may be added to the queue at any time, even if a prior analysis is in progress.
1. In the “Queue Mode” window, select “run individual sample” or “run batch of samples” and click “OK”, then follow the prompts. Use “batch sample” only if you want to use the same sample name and method for multiple vials.
2. You will be asked for your username and password.
3. Select an analysis method. Methods are posted on the GC oven door.
4. Place vial in the indicated position in the autosampler tray. To override the position assignment, click “cancel”, then enter the desired position.
5. Enter sample title and description, if desired
6. Check vial position
7. If you have only one sample, answer “no” to “Another Autoqueue?” If you have additional sample(s) to run, click “yes” and repeat steps 2-5.
8. Click on the “Report Manager” in the taskbar, and check the status of the instrument by double clicking on the “MS-1” line. If the window on the top right reads “status standby”, click on the “resume” button and enter “osesame” at the password prompt.
9. In Report Manger, be sure to note the filename that has been assigned to your sample; it consists of “P” followed by 4 numerals, then “.D”, e.g. “P1024.D”.
10. When a run is finished, a report will be printed automatically. The report consists of the total ion chromatogram (TIC), percent report, and library search results. If you want printouts of the mass spectra, they can be obtained through the Data Analysis module.
DURING THE RUN
• No need to wait around; you can come back later to pick up your printouts.
• If you want to watch the data as it is generated, click on “GC/MS Instrument #1” and maximize the “Total Ion Chromatogram” window. If the peaks go off-scale at the default attenuation (2.0E6), then your sample is too concentrated.
• Time elapsed/remaining can be viewed on the GC: press “time” on the GC keypad (top gray key on the far left)
• Use the standalone Data Analysis software module to take snapshots, etc.
ANALYZING DATA
1. If the “Enhanced Data Analysis” module is not already in the taskbar, a shortcut to the program (“Instrument #1 Data Analysis”) is located in the Windows XP Start menu.
2. Enter the same username and password as previously.
3. Select Load under the File menu
4. Select the data file, and the chromatogram will be displayed.
5. To expand a portion of the chromatogram or mass spectrum window, use the left mouse button to click and drag the cursor over the region of interest.
6. To zoom out to the entire range, double click the left mouse button.
7. To display the mass spectrum of a peak, place the pointer over the peak and double-click the right mouse button.
8. To display an averaged mass spectrum across a peak, click and drag the right mouse button across the region of interest.
9. To search the MS library, double click the right mouse button on the mass spectrum of a compound. (“Wiley275” must be selected as the library; this setting is in the “select library” option in the “Spectrum” menu.)
10. To print your data, select Print from the File menu. Either or both the TIC and MS windows may be printed. To generate .pdf files, select “Adobe PDF” as the printer.
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